User:Helen L. Slucher/Notebook/CHEM 571/2013/10/15

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Objective

Make HRP-AuNPs for enzymatic studies

Procedure

  • HRP-AuNP Solutions
  1. 30:1 Au:HRP
    1. 5.10 mL HRP and 850.34 μL Au
  2. 60:1 Au:HRP
    1. 2.56 mL HRP and 850.34 μL Au
  3. 90:1 Au:HRP
    1. 1.71 mL HRP and 850.34 μL Au
  4. 120:1 Au:HRP
    1. 1.28 mL HRP and 850.34 μL Au
  • The final volume was 10 mL, diluted with water
  • Synthesis of HRP-AuNPs
  1. Each solution was placed in a test tube
  2. The test tubes were placed in the oven at 80°C for approximately 3 hours

Notes

  • Stock solution of Au: 2.94 mM.
  • Stock solution of HRP: 16.27 μM.