Objective
Make HRP-AuNPs for enzymatic studies
Procedure
- 30:1 Au:HRP
- 5.10 mL HRP and 850.34 μL Au
- 60:1 Au:HRP
- 2.56 mL HRP and 850.34 μL Au
- 90:1 Au:HRP
- 1.71 mL HRP and 850.34 μL Au
- 120:1 Au:HRP
- 1.28 mL HRP and 850.34 μL Au
- The final volume was 10 mL, diluted with water
- Each solution was placed in a test tube
- The test tubes were placed in the oven at 80°C for approximately 3 hours
Notes
- Stock solution of Au: 2.94 mM.
- Stock solution of HRP: 16.27 μM.
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