# User:Helen L. Slucher/Notebook/CHEM 571/2013/09/04 Project name Main project page Previous entry      Next entry ## Overview

• Determine the molar absorptivities of adenosine and inosine.

## Data

1. Series 1: 0.4
2. Series 2: 0.8
3. Series 3: 1.6
4. Series 4: 2.4
5. Series 5: 3.2
6. Series 6: 4
7. Series 7: 4.8

## Calculations (Class Data)

1. Average absorbance = 0.250606061
2. Standard deviation = 0.152263534

The confidence level is calculated using the following equation:
$\displaystyle{ \left[ \bar{x} - \frac{ts}{\sqrt{n}}, \bar{x} + \frac{ts}{\sqrt{n}} \right] \, }$
90% confidence: [0.204,0.296]
95% confidence: [0.195,0.305]

• Inosine:
1. Average absorbance = 0.299666667
2. Standard deviation = 0.169381

90% confidence: [0.243,0.356]
95% confidence: [0.231,0.368]

$\displaystyle{ G = \frac{x_-\bar{x}}{s} }$

if Gcalc>Gtable, then the point is an outlier.
From the G-table, G=1.71.
Outliers:

1. (2.00E-05,0.406)
2. (2.50E-05,0.442)
3. (3.00E-05,0.558)

## Unknown

• Inosine unknown
1. Wavelength: 249 nm
2. Absorbance: 0.05
• After correcting the best fit line, Absorbance=11007*Concentration.
• 0.05=11007*Concentration
• Concentration = 4.54*10-6M
• Actual concentration = 2.37-5M.
• Percent error = 422.0%