Overview
- Determine the molar absorptivities of adenosine and inosine.
Data
- Series 1: 0.4
- Series 2: 0.8
- Series 3: 1.6
- Series 4: 2.4
- Series 5: 3.2
- Series 6: 4
- Series 7: 4.8
Class Data
Calculations (Class Data)
- Average absorbance = 0.250606061
- Standard deviation = 0.152263534
The confidence level is calculated using the following equation:
[math]\displaystyle{ \left[ \bar{x} - \frac{ts}{\sqrt{n}}, \bar{x} + \frac{ts}{\sqrt{n}} \right] \, }[/math]
90% confidence: [0.204,0.296]
95% confidence: [0.195,0.305]
- Average absorbance = 0.299666667
- Standard deviation = 0.169381
90% confidence: [0.243,0.356]
95% confidence: [0.231,0.368]
- [math]\displaystyle{
G = \frac{x_-\bar{x}}{s}
}[/math]
if Gcalc>Gtable, then the point is an outlier.
From the G-table, G=1.71.
Outliers:
- (2.00E-05,0.406)
- (2.50E-05,0.442)
- (3.00E-05,0.558)
Unknown
- Wavelength: 249 nm
- Absorbance: 0.05
- After correcting the best fit line, Absorbance=11007*Concentration.
- 0.05=11007*Concentration
- Concentration = 4.54*10-6M
- Actual concentration = 2.37-5M.
- Percent error = 422.0%
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