User:Helen L. Slucher/Notebook/CHEM-572/2014/03/04

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Objective Determine concentration of HRP GNPs Find equal amount of reactant-free GNPs UV/Vis Ran HRP GNPs from the alpha and beta methods The concentration of GNPs was determined to be around 10^12 M Data Series 1: Alpha method Series 2: Beta method Gamma Method (GNPs) 10^-8 M protein concentration 9.9 mL and 100 uL of protein Stir for 30 minutes at room temperature Spin for 30 minutes at 6500g Notes Concentrations Alpha and Beta: 10^12 M Gamma (reactant-free): 10^11, diluted to 10^10 M