Objective
- Determine concentration of HRP GNPs
- Find equal amount of reactant-free GNPs
UV/Vis
- Ran HRP GNPs from the alpha and beta methods
- The concentration of GNPs was determined to be around 10^12 M
Data
- Series 1: Alpha method
- Series 2: Beta method
Gamma Method (GNPs)
- 10^-8 M protein concentration
- 9.9 mL and 100 uL of protein
- Stir for 30 minutes at room temperature
- Spin for 30 minutes at 6500g
Notes
- Alpha and Beta: 10^12 M
- Gamma (reactant-free): 10^11, diluted to 10^10 M
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