User:Hector Medina/Notebook/iGEM 2010 Personal Log/2010/08/18

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Modelling & Dimensions

  • Amhed and I had a chat about the LED characterization. He found some very interesting papers discussing photon flux and absorbed photon flux. The conclusion we came to is the following:
    • Part of the current issues with modelling our system, is characterizing our light receptors. We've found a great protocol that can translate activator concentration into PopS (Polymerases per Second). However, since our activators are photons, we need something equivalent to photon concentration. We think that what interests us is the Absorbed (spectral) Photon Flux since that sounds like the effective activator concentration.
    • To obtain that, we need the Photon Flux.
    • And to get that, we plan on using this approach. Now the issue, is we need to measure our light source and obtain from that the watt/cm.
    • We were thinking of measuring luxes and transforming them to wat/cm with something like this. However, we still don't know how to determine the relation between both numbers at a wavelength other than 555nm...

I fear it has something to do with Integral Transforms, however without knowing the Kernel, how can we determine it? Moreover, since we are planning on using different filters (eg: blue, dark blue, really dark blue...) for our LED array, we must be able to make the measurement: ie: we can't determine it by the LED's specification. Grrr...