User:Hector Medina/Notebook/iGEM 2010 Personal Log/2010/06/16

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Parameters

  • Searching for the Kcat of the luciferin regeneration mechanism, I found a value of interest: 0.1 mM of oxyluciferin incubated with LRE and 0.5 mg of Luciferase yielded a quantifiable signal by luminometer. At least now we have a starting value for the assays.
    • doi:10.1074/jbc.M105528200
  • The activity of LRE appears to be 0.529
    • doi:10.1074/jbc.M105528200