User:Hattie Chung/Notebook/Intron Expression of mStaple/2010/01/19

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Entry title

  • Mini-prep pEYFP-2A-Kan from Patrick (Entry vector)
  • PCR 1.1 and PCR 2
  • Digest entry vector with AgeI
  • Make two agarose gel (1%)
  • Run gel, turn on heat block to 53C
  • Purify bands

Have: PCR1.1, PCR2, AgeI-vector

  • Digest entry vector with NotI
  • PCR 1.2
  • Clean PCR 1.2
  • Digest PCR 1.2 and PCR2 with XhoI