User:Hana Benzeer/Notebook/SGM Summer Project/2011/06/29

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Check overnight growth (4x200ml) Part strain Growth Colonies BBa_I15010 XL-1-Blue No NA BBa_I15010 DH5-α No Yes 1 BBa_I15008 XL-1-Blue Yes 66 BBa_I15008 DH5-αNo Yes 48 inoculation 2 parts to inoculate using transformed DH5-α 2 10ml tubes for each (4 total) Label the tubes Turn on the flame I15008, add 50μL,Ampicillin I15010, add 50μL, Kanamycin Inoculate 2 plates into each 2x 10 ml tubes. 4 tubes are then left in the shaker overnight Check overnight growth (10x100ml) Part strain Growth BBa_I13453 XL-1-Blue yes BBa_B0034 XL-1-Blue Yes BBa_I15009 XL-1-Blue Yes BBa_R0040 XL-1-Blue Yes BBa_B0015 XL-1-Blue Yes Take the tubes. Remove 700μL from each tube and transfer into eppendorf tube. Add 300μL of 50% glycerol stock into each eppendorf tube. Mix by vortex. Store in -21°C freezer. Spin the 10ml tubes for 10 min at 70. Remove the supernatant Store the tubes in -21°C freezer.