User:Hana Benzeer/Notebook/SGM Summer Project/2011/06/14

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Check overnight growth (4x10ml)

Colony Growth
Part Growth
BBa_E0840 Yes
BBa_E0840 Yes
BBa_R0082 Yes
BBa_R0082 Yes

Check transformation

  1. BBa_E0840, xx
  2. BBa_M30109, xx
  3. BBa_M30109, xx
  4. BBa_R0082, xx

Glycerol stock

For each of the 4 10ml bottles, use an eppendorf tube

  1. Add 700ul broth
  2. Add 300ul 50% Glycerol

Miniprep - Plasmid Purification

For all 4 10ml bottles

  1. Spin down and remove