User:Hana Benzeer/Notebook/SGM Summer Project/2011/06/06

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Preparing LB agar

  1. 6.4 g LB agar powder
  2. Add distilled water for a final volume of 200ml
  3. Autoclaving at 121°C for 15 min

Agar flask.jpg

Preparing selective plates

  1. 200ml of LB Agar
  2. Add 200 µl of Ampicillin
  3. Add 400 µl of Chloromphenical
  4. Pour 20ml media into each of 10 petri dishes

Selective plate.Jpg