User:Floriane Briere/Notebook/CHEM-496/2012/04/18

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Here are our FTIR data we obtained this semester; due to technical problems, we haven't been able to collect all data we wanted, especially we miss the FTIR spectra for the dye alone and for 166 and 70 ratio controls (without dye).

FTIR Results

  • BSA/HCl With Dye and BSA IR Spectra


  1. Peaks at 3400 are due to the stretching vibration of –OH in BSA
  2. Peak at 2350 is due to atmospheric carbon dioxide  this explains why there are shifts in all the values
  3. FTIR spectra of BSA alone in literature do not have these peaks
  4. To accurately identify the peak around 1100 in the BSA/HCl with dye, we would need to look at the IR spectra of just dye alone.
  5. It's possible that the peak at 1100 is due to the amine that forms when the dye bonds to the BSA
  • 70 and 166 Molar Ratio With Dye IR Spectra


  1. Other peaks characteristic of BSA and gold nanoparticles have been obscured by background
  2. Peaks at 2350 are due to interference by atmospheric carbon dioxide
  3. Peaks at 3350 are due to the stretching vibration of –OH in the BSA