User:Floriane Briere/Notebook/CHEM-496/2011/11/30

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Objective Today we are going to test our previous results; according to all our previous results the mole ratio of Au/BSA trigger purple fibers and purple solution formation. We are going to test two ratios (70 and 166); according to our previous results we are supposed to oberve fibers with the 166 ratio and we are supposed to obtain purple solution with the 70 ratio. Protocol BSA Stock Solution (17.7µM) 0.0117g BSA + 10mL water Ratio Gold/BSA = 70 (solutions A and B) 0.042mL of HAuCl4 (2.9mM) 0.1mL of BSA (17.7µM) 0.85mL of water Solution B at 80°C for 2 hours (constant temperature) Solution A removed every 30minutes from heat block and left 10 minutes at room temperature (discontinuous temperature) Ratio Gold/BSA = 166 (solutions D and C) 0.1mL of HAuCl4 (2.9mM) 0.1mL of BSA (17.7µM) 0.8mL of water Solution C at 80°C for 2 hours (constant temperature) Solution D removed every 30minutes from heat block and left 10 minutes at room temperature (discontinuous temperature) Results As a result we observed that: Solution A: we obtained a purple solution (with a 70 ratio and discontinuous temperature) Solution B: we obtained a purple solution (with a 70 ratio and constant temperature) Solution C: we obtained a clear solution with purple aggregate (all fibers are sticked together) (with a 166 ratio and constant temperature) Solution D: we obtained purple fibers spread through a clear solution (with a 166 ratio and discontinuous temperature)