User:Fermenter User/Notebook/STRY C1

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<!-- sibboleth --><div id="lncal1" style="border:0px;"><div style="display:none;" id="id">lncal1</div><div style="display:none;" id="dtext"></div><div style="display:none;" id="page">User:Fermenter User/Notebook/STRY C1</div><div style="display:none;" id="fmt">yyyy/MM/dd</div><div style="display:none;" id="css">OWWNB</div><div style="display:none;" id="month"></div><div style="display:none;" id="year"></div><div style="display:none;" id="readonly">Y</div></div>

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Background color codes:
Pastel Green: Major change of setup this time
yellow: Sung-Hye's correction or question to user

Letter color codes:
Black: Basic comment
Red: Emergency Information (Power shutdown, building access, Manager's absence etc)
Blue: Fermentation Setup information
Green: Discussion
Orange: Fermentation hrs

STRY C1


Day -1 (Apr 2 Wed):

   <Frank>               4/1/2009 13:20 Bring powder to fermenter room
   <Sung-Hye>            4/1/2009 11:30 Calibrate pH sensor
                         4/1/2009 13:30 Make 50-L media
                         4/1/2009 13:30 Add 10 ml of antifoam
                         4/1/2009 13:42 Start software
                         4/1/2009 13:50 Start Sterilization
                         4/1/2009 15:00 Cool down bioreactor
                                        300 rpm, 50-L/min air
                         4/1/2009 15:00 Connect Base (1000 ml) and Acid (1000 ml)
                         4/1/2009 15:00 Start pH control

Day 0 (Mar 24 The):

   <Sung-Hye>            4/2/2009 08:00 Connect Acid (1000 ml) and Base (1000 ml)
                         4/2/2009 08:00 Set pH
                         4/2/2009 08:00 Calibrate dO2 probe 
                         4/2/2009 08:00 Set dO2    
   <Frank>               4/2/2009 10:00  Add antibiotics
   <Frank>               4/2/2009 10:00  Fermentation 0:00
                                         Inoculation: (1000 ml) 
   <Frank>               4/2/2009 10:00  Fermentation 00:00
                                         Sampling (F4): OD (used as blank)
<Frank> 4/2/2009 12:10 Fermentation 02:10 Sampling (F4): OD (not measured)
<Sung-Hye> 4/2/2009 12:20 Fermentation 02:10 Change temperature <Frank> 4/2/2009 13:30 Fermentation 03:30 Sampling (F4): OD (0.4)
<Sung-Hye> 4/2/2009 14:30 Add inducer <Frank> 4/2/2009 00:00 Sampling Fermentation 00:00 F4: OD (??)

Day 1 (Apr 3 Fri):

   <Sung-Hye>            4/3/2009 07:00  Acid (980 ml) Base (800 ml)
   <Sung-Hye>            4/3/2009 09:30  Havest and Finish Fermentation



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