User:Fermenter User/Notebook/MOLD A11 F3

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Background color codes:
Pastel Green: Major change of setup this time
yellow: Sung-Hye's correction or question to user

Letter color codes:
Black: Basic comment
Red: Emergency Information (Power shutdown, building access, Manager's absence etc)
Blue: Fermentation Setup information
Green: Discussion
Orange: Fermentation hrs


Mold A11 (F3)

Day 0 (Oct 31th Fri):

      <Sung-Hye>           10/31/2008 (13:20)      Start Autoclave Cycle Started
      <Sung-Hye and Frank> 10/31/2008 (15:00)      Drain PBS, transfer media       
      <Sung-Hye>           10/31/2008 (15:02)      Start BioXpert software  Recording Time 0:00 
                                                   Starting rpm: 100 
      <Sung-Hye>           10/31/2008 (15:20)      Completion of Bioreactor setup for inoculation
<Frank> 10/31/2008 (17:15) Fermentation Time 0:00 Inoculation: ( 1,85*10^6/ml , 120 ml)


Day 1 (Nov 1st Sat):

      <Frank>              11/1/2008 (16:00)       Fermentation Time 22:45  
                                                   Cell counts: ( 0,12*10^6 /ml) Viability ( 95 %)


Day 2 (Nov 2nd Sun):

      <Frank>              11/2/2008 (15:00)       Fermentation Time 45:45  
                                                   Cell counts: ( 0,13*10^6 / ml) Viability ( 95 %)


Day 3 (Nov 3rd Mon):

      <Frank>              11/3/2008 (19:00)       Fermentation Time 73:45 
                                                   Cell counts: ( 0,11*10^6 / ml) Viability ( 95 %)


Day 4 (Nov 4th Tue):

      <Frank>              11/4/2008 (19:00)       Fermentation Time 97:45 
                                                   Cell counts: ( 0,15*10^6 / ml) Viability ( 95 %)


Day 5 (Nov 5th Wed):

      <Frank>              11/5/2008 (19:00)       Fermentation Time 121:45 
                                                   Cell counts: ( 0,23*10^6 / ml) Viability ( 95 %)
                           


Day 6 (Nov 6th Thu):

      <Frank>              11/6/2008 (15:15)       Fermentation Time 142:00 
                                                   Cell counts: ( 0,34*10^6 / ml) Viability ( 95 %)


Day 7 (Nov 7th Fri):

      <Frank>              11/7/2008 (16:10)      Fermentation Time 166:55 
                                                   Cell counts: ( 0,71*10^6 / ml) Viability ( 95 %)
                                                 
                                                  increased impeller speed to 200 rpm
                                                 

Day 8 (Nov 8th Sat):

      <Frank>              11/8/2008 (17:45)      Fermentation Time 192:30 
                                                   Cell counts: ( 1,01*10^6 / ml) Viability ( 90 %)
                                                 
                                                   increased dO2 to 200 ml/min
                                                 

Day 9 (Nov 9th Sun):

      <Frank>              11/9/2008 (16:30)      Fermentation Time 215:15 
                                                   Cell counts: ( 1,61*10^6 / ml) Viability ( 90 %)


Day 10 (Nov 10th Mon):

      <Frank>              11/10/2008 (14:20)     Fermentation Time 237:05 
                                                   Cell counts: ( 2,05*10^6 / ml) Viability ( 90 %)

 Between 237:06 (11/10/2008 14:20) and 262:15 (11/11/2008 15:30), sometihng happened to fluxuate pH and dO2.
 Did you change anything in terms of rpm or O2 flowrate?
 Did you find anything unsual (pressure of vessle, or condenser filter)?  
             

Day 11 (Nov 11th Tue):

      <Frank>              11/11/2008 (15:30)     Fermentation Time 262:15 
                                                   Cell counts: ( not determined / ml) Viability ( nd %)
                                                   cell are lysed.

Day 12 (Nov 12th Wed):

      <Frank>              11/12/2008 (13:50)     Fermentation Time 284:35 
                                                   Cell counts: (  nd / ml) Viability ( nd %)
      <Sung-Hye>           11/12/2008 (14:07)     Fermentation Time 284:52 
                                                   Terminate Fermentation


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