User:Fermenter User/Notebook/MACG G2

From OpenWetWare
Jump to: navigation, search

<!-- sibboleth --><div id="lncal1" style="border:0px;"><div style="display:none;" id="id">lncal1</div><div style="display:none;" id="dtext"></div><div style="display:none;" id="page">User:Fermenter User/Notebook/MACG G2</div><div style="display:none;" id="fmt">yyyy/MM/dd</div><div style="display:none;" id="css">OWWNB</div><div style="display:none;" id="month"></div><div style="display:none;" id="year"></div><div style="display:none;" id="readonly">Y</div></div>

Customize your entry pages Help.png

Background color codes:
Pastel Green: Major change of setup this time
yellow: Sung-Hye's correction or question to user

Letter color codes:
Black: Basic comment
Red: Emergency Information (Power shutdown, building access, Manager's absence etc)
Blue: Fermentation Setup information
Green: Discussion
Orange: Fermentation hrs
Pink: MeOH Induction hrs

MACG G2

Day -3 (Jul 24 Fri):

<Ganna>              7/24/2009 (00:00)     Prepare and autoclave additives:
                                           Prepare media (1-L/fermenter) with antifoam

Day 0 (Jul 27 Mon): == MeOH Induction Day 1

<Ganna>              7/27/2009 (00:00)     Take a colony from plate in 600 ml of BMGY(4 500ml flasks)    
                                           Allow it to gorw at 30°C.
<Ganna>              7/27/2009 (11:00)     Bring PBS bf(1-L/bioreactor) to fermenter room
<Sung-Hye>           7/27/2009 (11:00)     Calibratiron of pH sensor
                                           No Antifoam added (it is already in media prepared by Gunna)
                     7/27/2009 (13:30)     Autoclave start                          
                     7/27/2009 (17:28)     Start Fermenter #1 software
                     7/27/2009 (17:30)     Start Fermenter #2 software
                     7/27/2009 (17:32)     Start Fermenter #3 software
                     7/27/2009 (17:00-18:00)Media transfer
                     7/27/2009 (18:00)     Set rpm at 800 
                     7/27/2009 (18:00)     Start temp control  


<SungHye/Gunna>      7/27/2009 (18:30)     Fermentation 00:00  
                                           Inoculate cells into fermenters

Day 1 (Jun 28 Tue): == MeOH Induction Day 2


 Emergency building closure

Day 2 (Jun 29 Wed): == MeOH Induction Day 3

<SungHye/Gunna>      7/29/2009 (11:00)     Fermentation 40:30  
                                           MeOH calibration (5 ml 100% MeOH injection to 1-L media)
                     7/29/2009 (when?)     Fermentation 00:00 
                                           F1 (OD: ??  ) F2 (OD: ??  ) F3 (OD: ??  ) 
                     7/29/2009 (13:00)     Fermentation 44:30  
                                           Connect MeOH pump (F1)(F2)
                     7/29/2009 (13:15)     Fermentation 44:45 
                                           F1: Add Sorbitol
                                           F3: Add Casamino acid + Sorbitol 
                     7/29/2009 (15:30)     Fermentation 47:00  
                                           Connect MeOH pump (F3)

Day 3 (Jun 30 Thu): == MeOH Induction Day 4

<SungHye/Gunna>      7/30/2009 (13:00)     Fermentation 68:30  
                                           F1 (OD: ??  ) F2 (OD: ??  ) F3 (OD: ??  )    
                                           F1 (MeOH: ml) F2 (MeOH: ml) F3 (MeOH: ml)

Day 4 (Jun 31 Fri): == MeOH Induction Day 4

<SungHye/Gunna>      7/31/2009 (13:00)     Fermentation 92:30  
                                           F1 (OD: ??  ) F2 (OD: ??  ) F3 (OD: ??  )
                                           Harvest






Recently Edited Notebook Pages