User:Fermenter User/Notebook/MACG F12

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<!-- sibboleth --><div id="lncal1" style="border:0px;"><div style="display:none;" id="id">lncal1</div><div style="display:none;" id="dtext"></div><div style="display:none;" id="page">User:Fermenter User/Notebook/MACG F12</div><div style="display:none;" id="fmt">yyyy/MM/dd</div><div style="display:none;" id="css">OWWNB</div><div style="display:none;" id="month"></div><div style="display:none;" id="year"></div><div style="display:none;" id="readonly">Y</div></div>

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Background color codes:
Pastel Green: Major change of setup this time
yellow: Sung-Hye's correction or question to user

Letter color codes:
Black: Basic comment
Red: Emergency Information (Power shutdown, building access, Manager's absence etc)
Blue: Fermentation Setup information
Green: Discussion
Orange: Fermentation hrs
Purple: Induction hrs


MACG F12

Day -1 (Jan 13 Wed)

   <Ann>                 1/11/2010 (14:53)  Prepare small culture for inoculum (20mL)
   <Sung-Hye>            1/13/2010 (14:00)  Pour media (850 ml)
                         1/13/2010 (14:00)  pH calibration
                                            No Antifoam added
                         1/13/2010 (14:30)  Autoclave bioreactor                                             
                                            4 empty bottles/fermenter
                                            Base, Carbon source+Inoculation, Metal ions, Feeding

1/13/2010 (15:40) Cooling & Start Temp Control 1/13/2010 (15:40) Start F1 software 1/13/2010 (15:41) Start F2 software 1/13/2010 (15:42) Start F3 software 1/13/2009 (16:00) Calibrate dO2 1/13/2009 (16:00) Set dO2


   <Ann>                 5/12/2009 (22:23)  Prepare small culture for inoculum (200mL)

Day 0 (Jan 12 Tue)

   <Sung-Hye>            1/12/2010 (07:30)  Add carbon source using pumps  
                         1/12/2010 (10:00)  Connect Base (400 ml)
                                            Prime the pumps
                                            Set pH
   <Ann>                1/12/2010 (07:50)   Fermentation 00:00 
                             Inoculation 50 ml (F1, F2, F3)
   <Ann>                1/12/2010 (07:50)  Fermentation 00:00 
                             F1: OD (0.269) Contamination? (N)
                             F2: OD (0.270) Contamination? (N)
                             F3: OD (0.263) Contamination? (N)
   <Ann>                1/12/2010 (10:00)  Fermentation 02:10 
                             F1: OD (0.365) Contamination? (N)
                             F2: OD (0.384) Contamination? (N)
                             F3: OD (0.360) Contamination? (N)
   <Ann>                1/12/2010 (10:25)  Fermentation 02:35 
                             F1: OD (0.403) Contamination? (N)
                             F2: OD (0.418) Contamination? (N)
                             F3: OD (0.396) Contamination? (N)
   <Ann>                1/12/2010  (10:40)  Fermentation 02:50 Induction 00:00 
                             F1: Induce with Galactose (55 mL)  water (55 mL)
                                 Start feeding: (230uL/min)
                             F2: Induce with Galactose (55 mL)  supplement C1 (55 mL)                                   
                                 Start feeding: (230uL/min)
                             F3: Induce with Galactose (55 mL)  supplement C3 (55 mL)
                                 Start feeding: (230uL/min)


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