User:Fermenter User/Notebook/BROM D3

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<!-- sibboleth --><div id="lncal1" style="border:0px;"><div style="display:none;" id="id">lncal1</div><div style="display:none;" id="dtext"></div><div style="display:none;" id="page">User:Fermenter User/Notebook/BROM D3</div><div style="display:none;" id="fmt">yyyy/MM/dd</div><div style="display:none;" id="css">OWWNB</div><div style="display:none;" id="month"></div><div style="display:none;" id="year"></div><div style="display:none;" id="readonly">Y</div></div>

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Background color codes:
Pastel Green: Major change of setup this time
yellow: Sung-Hye's correction or question to user

Letter color codes:
Black: Basic comment
Red: Emergency Information (Power shutdown, building access, Manager's absence etc)
Blue: Fermentation Setup information
Green: Discussion
Orange: Fermentation hrs

BROM D3


Day 0 (Mar 11 Wed):

   <Sung-Hye>            3/11/2009 12:30 Assemble bioreactor 
   <Frances/Vincent>     3/11/2009 13:00 Bring media to fermenter room
                         3/11/2009 13:00 Add 5 ml of Antifoam
   <Sung-Hye>            3/11/2009 13:13 Start Sterilization (Liq 45 min)
                         3/11/2009 14:46 Cooling of bioreactor (Temp setup)
                         3/11/2009 14:46 Purge with 0.5 L/min O2 
                         3/11/2009 14:51 Start Fermentation Software for Fermenter #6 Record 0:00  
                         3/11/2009 15:00 Connect Acid (500 ml) and Base (400 ml), prime pumps
                                         Adjust pH to 7.0
                                         F6: Base: 400 ml - 250 ml = 150 ml (total consumption)
                         3/11/2009 15:05 Calibrate dO2 probe with 300 rpm, O2 purge for 30 min                    
                         3/11/2009 15:05 Set dO2
                         3/11/2009 15:05 Start pH control 
                         3/11/2009 15:05 Completion of bioreactor set up: Ready for inoculation
                                         rpm set to 400 dO2 <40%
  -------------------------------------------------------------------------------------------------------
   <Frances/Vincent>     3/11/2009 00:00 Inoculation: (?? ml) Fermentation 0:00

Day 1 (Mar 12 Thu):

   <Sung-Hye>            3/12/2009 (07:30) F6: Acid (480 ml) Base (1500 ml)
                                           Increase rpm to 600
                                           Bioreactor picture taken
<Frances/Vincent> 3/12/2009 (09:10) Sampling Fermentation 00:00 F6: Cell weight (109.8 mg/ml), Activity ( 65%, x100), Activity ( 16%, x1000)
<sung-Hye> 3/12/2009 (13:30) Increase rpm to 800 <Frances/Vincent> 3/12/2009 (14:00) Sampling Fermentation 00:00 F6: Cell weight ( 191.8 mg/ml), Activity ( 86%, x100), Activity (43 %, x1000)
<Sung-Hye> 3/12/2009 (15:00) F6: Base (130 ml)+ 200 ml (330 ml)

Day 2 (Mar 13 Fri):

   <Sung-Hye>            3/13/2009 (07:45) F6: Acid (410 ml) Base (310 ml)
   <Frances/Vincent>     3/13/2009 (09:00) Sampling Fermentation 00:00
                                            F6: Cell weight ( 245.6mg/ml), Activity ( 92%, x100), Activity (59 %, x1000)
<Frances/Vincent> 3/13/2009 (11:10) Sampling Fermentation 00:00 F6: Cell weight ( 288.2mg/ml), Activity ( 93%, x100), Activity ( 57%, x1000) <Frances/Vincent> 3/13/2009 (14:00) Sampling Fermentation 00:00 F6: Cell weight ( 289.4/ml), Activity ( 95%, x100), Activity ( 64%, x1000)
<Frances/Vincent> 3/13/2009 (00:00) Fermentation 00:00 <Sung-Hye> 3/13/2009 (15:00) Havest and Finish Fermentation F6: Acid (400 ml) Base (300 ml)




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