User:Fermenter User/Notebook/BROM C4

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<!-- sibboleth --><div id="lncal1" style="border:0px;"><div style="display:none;" id="id">lncal1</div><div style="display:none;" id="dtext"></div><div style="display:none;" id="page">User:Fermenter User/Notebook/BROM C4</div><div style="display:none;" id="fmt">yyyy/MM/dd</div><div style="display:none;" id="css">OWWNB</div><div style="display:none;" id="month"></div><div style="display:none;" id="year"></div><div style="display:none;" id="readonly">Y</div></div>

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Background color codes:
Pastel Green: Major change of setup this time
yellow: Sung-Hye's correction or question to user

Letter color codes:
Black: Basic comment
Red: Emergency Information (Power shutdown, building access, Manager's absence etc)
Blue: Fermentation Setup information
Green: Discussion
Orange: Fermentation hrs

BROM C4 (F7)


Day 0 (Jun 1 Mon):

Change #1: Media composition: JR
   <Natasha>             6/1/2009 (10:30) Bring media to fermenter room
<Sung-Hye> 6/1/2009 (11:00) Assemble bioreactor 6/1/2009 (11:00) Add 10 ml of Antifoam 6/1/2009 (11:10) Start Sterilization (Liq 45 min) 6/1/2009 (12:50) Cooling of bioreactor (Temp setup) 1 L/min air, 400 rpm 6/1/2009 (12:55) Start Fermentation Software for Fermenter #7 6/1/2009 (13:05) Connect Acid (250 ml) and Base (340 ml after prime pump and bring pH to 7.0) Start pH control

Day 1 (Jun 2 Tue):

                         6/2/2009 (09:00) Calibrate dO2 probe with 250 rpm, O2 purge for 30 min
                         6/2/2009 (09:00) Set dO2
                         6/2/2009 (09:00) Completion of bioreactor set up: Ready for inoculation


   <Natasha>             6/2/2009 (11:15) Inoculation: inoculation volume (300 ml) Fermentation 0:00

<Natasha> 6/2/2009 (11:20) Sampling Fermentation 0:05 Cell weight (10 mg), Activity (65%, x100), Activity (25% , x1000) <Natasha> 6/2/2009 (17:55) Sampling Fermentation 6:35 Cell weight (77 mg), Activity (92%, x100), Activity (65%, x1000)

Day 2 (Jun 3 Wed):

   <Sung-Hye>            6/3/2009 (8:15) Fermentation 09:00
                                           Replace O2 tanks
   <Natasha>             6/3/2009 (9:30) Sampling  Fermentation 22:05
                                           Cell weight ( 156 mg), Activity (  90%, x100), Activity (   77 %, x1000)
   <Vincent/Natasha>     6/3/2009 (10:45) Fermentation 11:30
                                           Harvest Fermentation






Recent changes

19 October 2017

     14:13 

BME100 f2017:Group14 W0800 L4‎‎ (9 changes | history) . . (+9). . [Shae M. Diaz‎ (9×)]

     

14:13

(cur | prev) . . (+8). . Shae M. Diaz (talk | contribs) (Protocol)

     

14:12

(cur | prev) . . (-31). . Shae M. Diaz (talk | contribs) (TEAM MEMBERS)

     

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(cur | prev) . . (+31). . Shae M. Diaz (talk | contribs) (TEAM MEMBERS)

     

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(cur | prev) . . (+4). . Shae M. Diaz (talk | contribs) (TEAM MEMBERS)

     

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(cur | prev) . . (-1). . Shae M. Diaz (talk | contribs) (TEAM MEMBERS)

     

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(cur | prev) . . (+1). . Shae M. Diaz (talk | contribs) (TEAM MEMBERS)

     

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(cur | prev) . . (-1). . Shae M. Diaz (talk | contribs) (TEAM MEMBERS)

     

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(cur | prev) . . (+1). . Shae M. Diaz (talk | contribs) (TEAM MEMBERS)

     

13:55

(cur | prev) . . (-3). . Shae M. Diaz (talk | contribs) (TEAM MEMBERS)

 m   14:12  Swartz‎ (diff | hist) . . (+61). . Marcus Rohovie (talk | contribs)
     14:10 (Move log) . . Marcus Rohovie (talk | contribs) moved page Swartz to Swartz:Main

     14:08 

(Upload log). .

[Thorntca‎; Shae M. Diaz‎ (3×)]

     

14:08

. . Shae M. Diaz (talk | contribs) uploaded File:100profileshae.JPG

     

14:01

. . Shae M. Diaz (talk | contribs) uploaded File:Shaediaz100.JPG

     

13:51

. . Shae M. Diaz (talk | contribs) uploaded File:BME100ShaeD.JPG

     

13:17

. . Thorntca (talk | contribs) uploaded File:2Dgel.png.jpg

     14:06  Rich Lab:Funding‎ (diff | hist) . . (-24). . Isabel C. Morales (talk | contribs)

     13:52 

Klenow Assembly Method: Seamless cloning‎‎ (3 changes | history) . . (+101). . [David M.D. Bailey‎ (3×)]

     

13:52

(cur | prev) . . (-5). . David M.D. Bailey (talk | contribs) (The Klenow Assembly Method (KAM) for the seamless cloning of overlapping double stranded DNA fragments: Very cheap alternative to the Gibson Assembly)

     

13:51

(cur | prev) . . (+1). . David M.D. Bailey (talk | contribs) (The Klenow Assembly Method (KAM) for the seamless cloning of overlapping double stranded DNA fragments: Very cheap alternative to the Gibson Assembly)

     

13:50

(cur | prev) . . (+105). . David M.D. Bailey (talk | contribs) (The Klenow Assembly Method (KAM) for the seamless cloning of overlapping double stranded DNA fragments: Very cheap alternative to the Gibson Assembly)

     13:52 

User:Peteral‎‎ (2 changes | history) . . (+1,079). . [Peteral‎ (2×)]

     

13:52

(cur | prev) . . (+351). . Peteral (talk | contribs) (Alec's Discussion Questions)

     

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(cur | prev) . . (+728). . Peteral (talk | contribs) (Alec's Discussion Questions)

     13:22 

User:Thorntca‎‎ (8 changes | history) . . (+529). . [Thorntca‎ (8×)]

     

13:22

(cur | prev) . . (+145). . Thorntca (talk | contribs)

     

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(cur | prev) . . (+17). . Thorntca (talk | contribs)

     

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(cur | prev) . . (+4). . Thorntca (talk | contribs)

     

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(cur | prev) . . (+30). . Thorntca (talk | contribs)

     

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(cur | prev) . . (+417). . Thorntca (talk | contribs)

     

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(cur | prev) . . (+12). . Thorntca (talk | contribs)

     

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(cur | prev) . . (-5). . Thorntca (talk | contribs)

     

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(cur | prev) . . (-91). . Thorntca (talk | contribs)

     12:27 

User:Madeline.churchill‎‎ (2 changes | history) . . (+302). . [Madeline.churchill‎ (2×)]

     

12:27

(cur | prev) . . (0). . Madeline.churchill (talk | contribs) (TZAP protein controls telomere Trimming process)

     

12:27

(cur | prev) . . (+302). . Madeline.churchill (talk | contribs) (Madeline Churchill's Discussion Page)

     12:15  CONJ606:Materials‎ (diff | hist) . . (+319). . Weinjule (talk | contribs) (Papers)
     09:17  BioMicroCenter:BIG meeting‎ (diff | hist) . . (+19). . George W Bell (talk | contribs) (2017-2018 academic year)
     07:06 (User creation log) . . User account Newfish (talk | contribs) was created by Yar (talk | contribs) and password was sent by email ‎

     04:10 

BioMicroCenter:BioinfoClasses‎‎ (3 changes | history) . . (+132). . [Charlie Whittaker‎ (3×)]

     

04:10

(cur | prev) . . (+9). . Charlie Whittaker (talk | contribs) (Teaching Schedule)

     

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(cur | prev) . . (0). . Charlie Whittaker (talk | contribs) (Teaching Schedule)

     

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(cur | prev) . . (+123). . Charlie Whittaker (talk | contribs) (Teaching Schedule)

     03:36  User:Timothee Flutre‎ (diff | hist) . . (+146). . Timothee Flutre (talk | contribs) (add contrib breedR and SO)

 m   02:27 

How to write a research paper‎‎ (2 changes | history) . . (+91). . [Formand‎ (2×)]

 m   

02:27

(cur | prev) . . (-1). . Formand (talk | contribs) (External links)

 m   

02:24

(cur | prev) . . (+92). . Formand (talk | contribs) (External links)

18 October 2017

     19:09  BME100 f2017:Group10 W0800 L4‎ (diff | hist) . . (+1). . Osvaldo J Pagan (talk | contribs)
     15:56  BME100 f2017:Group8 W1030 L4‎ (diff | hist) . . (+409). . Jennifer L. Brodsky (talk | contribs) (Research and Development)
     15:20  BME100 f2017:Group7 W0800 L4‎ (diff | hist) . . (-4). . Miguel R. Almanza Lopez (talk | contribs) (OUR TEAM)

     15:16 

(Upload log). .

[Miguel R. Almanza Lopez‎; Kaifu Chen‎ (2×)]

     

15:16

. . Miguel R. Almanza Lopez (talk | contribs) uploaded File:MralPoopy.jpg

     

14:39

. . Kaifu Chen (talk | contribs) uploaded File:Jcm.jpg

     

14:38

. . Kaifu Chen (talk | contribs) uploaded File:Wgy.jpg

     14:40 

BME100 f2017:Group1 W1030 L4‎‎ (4 changes | history) . . (+459). . [Abby E. Krell‎ (4×)]

     

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(cur | prev) . . (+33). . Abby E. Krell (talk | contribs) (Protocol)

     

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(cur | prev) . . (+28). . Abby E. Krell (talk | contribs) (Protocol)

     

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(cur | prev) . . (+220). . Abby E. Krell (talk | contribs) (Protocol)

     

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(cur | prev) . . (+178). . Abby E. Krell (talk | contribs) (Protocol)