User:Eric M. Walters/Notebook/Spring 2012/2012/06/26

Cyanobacterial acrylate production

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The story so far Based on the gels yesterday, everything that came up on the 10 mM AA plates has more of the barcode-sized fragment than the acsA-sized. I am going to investigate a smaller number of clones with 3 PCR assays, though I can only do two today: BC-F + acsA screen R Ta = 52, extension = 1:00 (861 bp) acsA screen F + acsA screen R Ta = 50, extension = 3:50 (3883 bp) internal acsA F (submitted today) + acsA screen R Ta = 50, extension - 1:15 (1215 bp) I will run all of these with only 25 cycles (vs yesterday's 35), since the BC bands seemed to overwhelm some lower-copy elements in yesterday's gel.

Internal barcode + flanking gel