User:Eric M. Walters/Notebook/Spring 2012/2012/06/26
|Cyanobacterial acrylate production||Main project page|
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The story so far
Based on the gels yesterday, everything that came up on the 10 mM AA plates has more of the barcode-sized fragment than the acsA-sized. I am going to investigate a smaller number of clones with 3 PCR assays, though I can only do two today:
I will run all of these with only 25 cycles (vs yesterday's 35), since the BC bands seemed to overwhelm some lower-copy elements in yesterday's gel.
Internal barcode + flanking gel