User:Eric M. Walters/Notebook/Spring 2012/2012/06/08

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Yet another colony PCR on the acsA::barcode

Each 10 uL reaction:

  • 5 uL GoTaq 2x MM
  • 4 uL Nuclease free water
  • .5 uL fresh acsA-UpFv1 primer
  • .5 uL fresh acsA-DnRv1 primer

50 degrees annealing, 3 min extension (acsA is 2kb), 30 cycles so I don't drown out the deletion if it's less prevalent

Pflegerlab 2012-06-08 15hr 26min colony PCR of flanking primers for acsA;;barcode.jpg

1-9 are actually: 1, 2, 3, 5, 6, 8, 9, 10, 12