User:Eric M. Walters/Notebook/Spring 2012/2012/05/24

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Plating the transformations

  • Uh oh! Need acrylate plates, but they've all got kanamycin in them
  • Making 500 μM AA Media A plates (500 mL of 'em)
  • 500 μL of .5 M AA into 500 mL batch should give me 500 μM
  • 25 μL of B12, it's what cyanos crave

Plated the 2 mL (spun down and resuspended) on each (wt/ΔacsA