User:Eric M. Walters/Notebook/Spring 2012/2012/05/23

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ΔacsA::BC

Today I'm making sure I've got enough of the proper construct, then getting it into wt 7002.

Checking the PCR product

I cleaned up each 50 μL reaction separately and got 50 and 57 ng/μL Ran a single lane of a mix of unclean products of both reactions, seen here:

Pflegerlab 2012-05-23 10hr 34min acsA;;BC construct post-amplification.jpg

Transformation

  • The wt cultures was started 5/18 and is at OD730 0.48. Doubling OD by spinning down.
  • Added 60 μL at ~54 ng/μL, so 3,200 ng into the culture. Also had wt 7002 alongside. Put in light at 11:00