User:Eric M. Walters/Notebook/Spring 2012/2012/05/22
|Cyanobacterial acrylate production||Main project page|
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SphI-HF was used to digest, in Buffer 4 at 37°C, 45 minutes (10:45-11:30). Heat inactivated at 65°C for 20 minutes.
Ligation will be done for 30 minutes at room temperature (12:05-12:35), per Matt's advice. 50 μL, since that's what I've needed before.
Gel after 30 minutes (but continued for another 60):
I want to increase the amount of ligation produce I've got to transform with via PCR. 2x50 μL reactions
Per 50 μL:
Run overnight to be cleaned, AGEd, and transformed.