User:Eric M. Walters/Notebook/Spring 2012/2012/04/27

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  • I started liquid cultures of pMevT and pMBI on Tuesday to miniprep and make freezer stocks. I then forgot about the cultures and left them shaking until today. They're probably worthless, so I'll start some more cultures to at least make freezer stocks tomorrow.
  • Also, there are still no colonies on the acsA::acc plates. This could be due to acsA still being present, the kan resistance being lost, or because I spun them down too fast when I wanted to plate them.