User:Eric M. Walters/Notebook/Spring 2012/2012/03/05

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Assembly attempt 4(?): Digest

  • All three PCR products were shown to be proper size via gel
    • In attempts to keep high DNA yields, didn't clean/NanoDrop
  • Digesting 100ul of each and will set up big ligation later

Digest (Up/Down/AccBCDA):

  • EcoRI-HF (2/0/2)
  • BamHI-HF (0/2/2)
  • Buffer 4 (10/10/10)
  • PCR product (80/80/80)
  • H2O (8/8/6)

From 1:45 to 4:15 (2.5 hours) and onto ice to be gel extracted after iGEM class.