User:Emmalee Jones/Notebook/Lab Notebook/2010/04/30

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Finding Even Coverage Concentration

  • Made 0.1 mM total lipid dilution in PEM
  • Used this to make lipid flow cell solution
  • Still not covering the entire slide, so made up a new solution that is 0.5 mM, put in a flow cell
  • This one has even coverage! So that is when I make a 0.5 mM dilution in PEM, then put that into a half and half lipid motility solution for the flow cell. I only made a half batch of it, and that was plenty to run a few flow cells.

Half batch:

    25 μL liposomes
    1.25 μL AF
    0.5 μL Glucose
    23.3 μL PEM