User:Emmalee Jones/Notebook/Lab Notebook/2010/03/15

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Extruded vesicles

  • Extruded vesicles using the 30% DMPG lipid solution
  • Diluted 20 μL of the vesicles in PBS buffer
  • Used DLS to measure size
  • First batch I got:


First Run
Rh=113.46 ± 6.13 nm
Pgpctrday1cumulants.JPG

Subpeak 1 2 3
Rh mean 7.22 90.71 153778.42
Rh peak 7.39 91.21 133272.67
Rh StDev 0.73 19.03 38251.54
Peak Area % 1.14 95.72 3.14
Pgpctrday1reg.JPG


Second Run
Rh=114.33 ± 5.26 nm
Pgpctrb2day1cumulants.JPG

Subpeak 1 2
Rh mean 5.94 116.58
Rh peak 5.88 119.90
Rh StDev 0.53 50.44
Peak Area % 0.43 99.57

Pgpctrb2day1reg.JPG
Second batch I got:
First run
RH = 73.85 ± 6.35 nm
Pgpctrb3day1cumulants.JPG

Subpeaks 1 2
Rh mean 2.93 72.70
Rh peak 2.84 74.50
Rh Std Dev 0.47 25.95
Peak Area % 1.11 98.89


Pgpctrb3day1reg.JPG
Second Run
Rh = 80.08 ± 5.45 nm
Pgpctrb4day1cumulants.JPG

Subpeaks 1 2
Rh mean 3.28 77.70
Rh peak 2.89 75.87
Rh Std Dev 0.48 31.14
Peak Area % 0.71 99.29
Pgpctrb4day1reg.JPG