Extrusion and DLS of new sample
- Extrusion process went smoothly today.
- I looked at the vesicles with DLS. NOTE: I should have saved the little beaker of PBS so I didn't have to pour more out.
- Results were very similar to the results from the batch from Friday, surprisingly. So that means the Friday batch hadn't decayed yet I guess. Next up is to make some with 30% PG, and then add in Texas Red.
- I want to see if I get a similar size for the part PC part PG vesicles. If I do . . . that is pretty small. I'll have to check if I can see them at all.
First Batch:
Cumulants
RH - 64.18 ± 3.51 nm
Correlation Function
Regularization
Peak | 1 | 2 |
Max | 3.84 | 66.56 |
Mean | 3.93 | 62.42 |
Min | 0.69 | 27.84 |
Area % | 1.34 | 98.66 |
Second Batch
Cumulants
RH - 68.79 ± 4.71 nm
Regularization
Peak | 1 | 2 |
Max | 3.71 | 63.70 |
Mean | 3.74 | 59.39 |
Min | 0.61 | 23.04 |
Area % | 1.65 | 98.35 |
I did a few more tests, with similar results
|