User:Emmalee Jones/Notebook/Lab Notebook/2010/02/17

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Working in CBME Lab

To prepare lisosomes using the desiccator I measured out:

  • 250 μL of 20 mg/mL egg PC
  • 750 μL of chloroform to make solution 5 mg/mL

Put in desiccator with fresh crystals to sit at least overnight.

Sidenote about refreshing desiccator crystals

Tried to put crystals in oven to refresh, but apparently they smell really bad while they are doing so . . . Oops. So since I didn't need them finished today I turned off the oven. Someone from Dr. Dirk's lab said they had a vacuum oven which I could use overnight, so I put the crystals in there.