User:Emmalee Jones/Notebook/Lab Notebook/2010/02/12

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Making PEM Buffer Today I made a batch of PEM Buffer. The ingredients for 250 mL of PEM Buffer at pH 6.89 are 6.074 g of PIPES to get 80 mM .0951 g of EGTA to get 1mM .25 mL of MgCl2 1 M solution to get 1mM 30.125 mL of NaOH 1 M solution to get ~125 mM These are important things for me to remember: - Clean glassware is over the Autoclave - DON'T put pipettes in stock solutions - When using the pH meter don't put cap on before filling with the storage solution and recovering hole with tape - To clean a spatula (scooper tool) use soapy sponge, rinse in sink, then rinse with MilliQ (18.2 ohms resistance) water, then rinse with alcohol, then rinse with MilliQ again - When measuring out ingredients, the scale takes a long time to come to the right weight