User:Elizabeth Ghias/Notebook/Experimental Biological Chemistry/2012/01/31

From OpenWetWare
Jump to: navigation, search
BDLlogo notext lr.png Experimental Biological Chemistry Report.pngMain project page
Resultset previous.pngPrevious entry      Next entryResultset next.png


To create stock solutions for the reactants used to produce gold nanoparticles and to decide which dye should be used for nanoparticle functionalization.


  1. Make 40 mL of a 17.7 uM stock solution of BSA by mixing 0.0468 g BSA with 40 mL water.
  2. Make 20 mL of a 2.9 mM stock solution of HAuCl4 by mixing 0.0197 g HAuCl4 with 20 mL water.
  3. Selected a dye from Marker Gene Technologies Inc.


No data was collected today


  • The dye that will be used must either have a red shift or blue shift from the absorbance wavelength of the gold nanoparticles and must contain a succinimidyl group.
  • The dye selected is 5(6)-Carboxynapthofluorescein, N-succinimidyl ester (C33H19NO9. This dye is a long wavelength fluorophore that reacts with terminal and internal lysine residues at a pH of 8-9. Its fluorescence is pH dependent. It absorbs at 602 and emits at 672 nm.