User:Elizabeth Ghias/Notebook/Experimental Biological Chemistry/2011/10/04

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To determine the sensitivity of a fluorimeter by using standard concentrations of RU(bpy)(phen) in acetonitrile


  1. 3 mL standards of Ru(bpy)(phen) in acetonitrile were prepared with concentrations ranging from 0 to 35 μM
  2. The solutions were placed in the fluorimeter and the intensities were determined.


The following concentrations of Ru(bpy)(phen) and acetonitrile were used to prepare the standards. October 4 Ru(bpy)(Phen) concentrations.png

The spectrum of intensities was graphed for each concentration. The intensities recorded by the fluorimeter were corrected with the intensities for pure acetonitrile.

October 4 Ru(bpy)(phen) standard intensities.png

Excel was then used to determine the integrated intensities of each standard.

October 5 Integrated Intensities.png

October 5 Integrated Intensities vs. concentration.png


The color of the Ru(bpy)(phen)solutions became increasingly more orange as the concentrations increased.

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