User:Elizabeth Ghias/Notebook/Experimental Biological Chemistry/2011/09/06

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To form zein protein filaments through the use of BSA conjugated nanoparticles


  1. A 90% ethanol solution with a total volume of 10 mL was made by mixing 9 mL or ethanol with 1 mL of H2O.
  2. A solution for the film containing zein (10% w/v) and glycerol (30% of zein weight) was made by mixing 1 g of zein and 0.3 mL of glycerol.
  3. The 90% ethanol solution was added to the film so that the total volume was 10 mL. The solution was mixed by pipetting.
  4. 5 grams of the solution was placed on the bottom of a labeled Petri dish with a 9 cm diameter.
  5. The Petri dish was placed in an oven set to 37°C for 24 hours.


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Film after 24 hours in the oven.


The density of the glycerol was used to determine the volume of glycerol that was added to the zein protein.

0.3 g glycerol x 1 ml/1.261 g = 0.238 ml glycerol