User:Eleni N. Kalivas/Notebook/CHEM-571/2015/01/30

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January 30, 2015

  • Malachite Green calibration curve
  • redo PVA-Alg/PVA-Alg-NaMT bead synthesis, same scale, 4% CaCl, smaller beads using glass pipette

Procedure

Procedure adapted from | Kuo-Ying Amanda Wu and Keith D. Wisecarver (1992)<br.> Modified from 1/28/2015

  1. Prepare an aqueous solution of 13% PVA and 0.02% sodium alginate at 60°C (Stir plate on 3rd setting: ~68°C)
    • 1 gram of PVA and 0.16 g of sodium alginate in 7 mL of water (12.5% PVA and ~0.02% alginate)
      • Add alginate while stirring PVA-water solution
    • ~0.1 g of nanocomposites should be added to this mixture where applicable
  2. Cool dissolved and mixed solution to 35°C
  3. Prepare 210 mL of saturated citric acid with 2% calcium chloride
    1. Add 8.4 grams (4%) of calcium chloride to 210 mL of water
    2. Add citric acid to saturation (32 g citric acid)
      • 14.7g/100mL > 30.87 g/210 for solubility comparable to boric acid
  4. Add the PVA-Alg-Nanocomposite mixture drop-wise (by syringe) to the gently stirring (stir plate 3) citric acid-calcium chloride solution using a Pasteur pipette
  5. Stir for 2 hrs to allow solidification
  6. Rinse with water to remove excess reactants

Measurements

  • PVA-Alg
    • PVA: 1.044 g
    • Alginate: 0.16 g
    • CaCl: 8.213 g
    • citric acid: 32.01 g
  • PVA-Alg-NaMT
    • PVA: 0.994 g
    • Alg: 0.156 g
    • NaMT: 0.103 g
    • CaCl: 8.381 g
    • citric acid: 33.01 g
  • PVA-Alg-NaMT #2
    • PVA: 1.098 g
    • Alginate: 0.159 g
    • NaMT: 0.125 g
    • CaCl: 8.9 g
    • citric acid: 32.02 g
    • CaCl: 8.403 g
    • citric acid: 32.04 g
  • Vial, no cap: 13.023 g

Notes

  • PVA-Alg does not work
  • PVA-Alg-NaMT #2 made in sonicator

Next Week

  • Madeleine: MG Calibration curve
  • Eleni: Scale up PVA-Alg-NaMT synthesis
  • James: start next nanocomposite synthesis