Objective
To begin collecting all data and based on our results refine the protocol for the rest of the semester.
Table of Volumes for Large Synthesis
80% BSA-Cysteine
% BSA
|
5 mM Zinc Nitrate (uL)
|
5mM Manganese Acetate (uL)
|
40 mM Ammonium Acetate (uL)
|
10uM BSA* (uL)
|
10uM 1-Thioglycerol (uL)
|
10mM Ammonium Hydroxide (uL)
|
5 mM Sodium Sulfide* (uL)
|
Total Volume (mL)
|
80 |
10800 |
1200 |
12000 |
9600 |
2400 |
60000 |
12000 |
108
|
80% BSA-Cysteine, half concentration
% BSA
|
2.5 mM Zinc Nitrate (uL)
|
2.5 mM Manganese Acetate (uL)
|
20 mM Ammonium Acetate (uL)
|
5 uM BSA* (uL)
|
5 uM L-Cysteine (uL)
|
5 mM Ammonium Hydroxide (uL)
|
2.5 mM Sodium Sulfide* (uL)
|
Total Volume (mL)
|
80 |
10800 |
1200 |
12000 |
9600 |
2400 |
60000 |
12000 |
108
|
80% BSA-GSH
% BSA
|
5 mM Zinc Nitrate (uL)
|
5mM Manganese Acetate (uL)
|
40 mM Ammonium Acetate (uL)
|
10uM BSA* (uL)
|
10uM GSH (uL)
|
10mM Ammonium Hydroxide (uL)
|
5 mM Sodium Sulfide* (uL)
|
Total Volume (mL)
|
80 |
10800 |
1200 |
12000 |
9600 |
2400 |
60000 |
12000 |
108
|
80% BSA-1-Thioglycerol
% BSA
|
5 mM Zinc Nitrate (uL)
|
5mM Manganese Acetate (uL)
|
40 mM Ammonium Acetate (uL)
|
10uM BSA* (uL)
|
10uM 1-Thioglycerol (uL)
|
10mM Ammonium Hydroxide (uL)
|
5 mM Sodium Sulfide* (uL)
|
Total Volume (mL)
|
80 |
10800 |
1200 |
12000 |
9600 |
2400 |
60000 |
12000 |
108
|
Preparation of Stock Solutions
'
|
mass in grams (g)
|
Final Volume (mL)
|
5mM Zinc Nitrate |
0.0745 |
50
|
5mM Manganese Acetate |
0.0123 |
10
|
40mM Ammonium Acetate |
0.1541 |
50
|
10uM BSA |
0.033 |
50
|
10mM Ammonium Hydroxide |
168.92 (uL) |
250
|
5mM Sodium Sulfide |
0.06 |
50
|
|