User:Eleni N. Kalivas/Notebook/CHEM-571/2013/09/04

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Overview

Due to time constraints we were not able to run a UV-vis for the inosine spectra. The objective for today includes analyzing yesterdays data in order to check the spectra and calibration curves and make any necessary corrections, and to also do the same for the inosine solutions.

Analysis

After looking over the data from yesterday there were multiple problems that needed correcting. First we noticed that our 0.5M adenosine was the exact same data as the water that was run as a blank. This lead us to believe that the data was saved incorrectly and that some of the dilutions may have just been off. We re-ran the 0.5M adenosine sample and got comparatively valid data. We then noticed that our 1.0M adenosine peaked below the 0.5M which indicated a clear error. Therefore we re-made the 1.0M adenosine and received much better data. Our last observation was that the 3.0M adenosine was too far off when analyzing the calibration curve, so we also re-made the 3.0M adenosine dilution and re-ran it and received much better data.

  • Preparation of stock solution

Adenosine stock preparation.png

  • Adenosine Dilutions

Adenosine Dilutions.png

  • Absorbance Spectra of Adenosine

Corrected aborsbance.png


  • Calibration Curve of Adenosine data

Screen Shot 2013-09-04 at 2.02.16 PM.png

Inosine

Once the Adenosine samples had been run and the spectra and the calibration curve had been corrected, the inosine samples were now being run. After running all the data the spectra was plotted and the calibration curve was made. Analysis of the calibration curve concluded.

  • Stock Solution

2013 Inosine Stock solution zem.png

  • Inosine Dilutions

2013 Inosine Dilutions.png

  • Absorbance spectra of Inosine

2013 insoine absorbance zem.png

  • Calibration curve of Inosine data

2013 zem inosine calibration curve.png

Group Calibration Data

  • Adenosine group calibration curve

Adenosine extinction coefficent.png

  • Inosine group calibration curve

Inosine extinction coefficent.png

  • Standard Deviations

the left is the standard deviation table of Adenosine, while that on the right is the standard deviation table of the Inosine data Adenosine and Inosine st. deviations ZEM.png

  • Confidence Interval at 95%

the left is for Adenosine and the right is for the Inosine data 95% CI 2103 zem.png

  • Confidence interval at 90%

the left is for Adenosine and the right is for Inosine 90% CI zem.png

  • Q-test at 90% confidence level

Adenosine: Since Qc=0.64, the third and fourth points can be discarded with 90% confidence as outliers A Qtest zem.png

Inosine: since Qc=0.64 the first four data points listed in the table can be discarded with 90% confidence as outliers I q-test zem.png

  • Grubbs test for Adenosine Absorbance Values

Adenosine Grubbs test.png

since Gc=1.67 the first three values, of 0.558, 0.442 and 0.406, listed on the table can be discarded with 95% confidence as outliers

Identifying an unknown Adenosine sample

2013 zem adenosine unknown.png

  • The concentration above was calculated to be 4.7344E-5 M based on the molar extinction coefficient determined from the Adenosine calibration curve, with a percent error in unknown concentration is 1%.