Objective
- determine the molar absorptivity of adenosine and inosine.
- these two molecule's functionality differ in that adenosine contains a primary amine while inosine contains a carboxy group
- Analyze changes in the UV-vis spectra to determine changes in the concentration.
- use Beer's law to determine the molar absorptivity, and create a calibration curve
Once each group has created a calibration curve we will then determine the standard deviations and the confidence interval, and lastly we will perform a Q-test in order to remove any outlining data.
Adenosine Protocol
Using 0.2006 grams of adenosine and a 50mL volumetric a stock solution of 0.015M adenosine solution was prepared. The stock solution was then diluted to 1.5e-4 M solution, which was used to create the following dilutions which was prepared to run on the UV-vis.
| Adenosine solution concentrations (M)
|
| 3.00x10-5
|
| 2.50x10-5
|
| 2.00x10-5
|
| 1.50x10-5
|
| 1.00x10-5
|
| 0.50x10-5
|
RANDOM
- The data was then analyzed for corrections which will be done tomorrow.
Inosine Protocol
Using 0.1347 grams of inosine and a 25mL volumetric a stock solution of 0.02M adenosine solution was prepared. The stock solution was then diluted to 4.8e-4 M solution, which was used to create the following dilutions which was prepared to run on the UV-vis.
| Inosine solution concentrations (M)
|
| 4.80x10-5
|
| 4.00x10-5
|
| 3.20x10-5
|
| 2.40x10-5
|
| 1.60x10-5
|
| 0.80x10-5
|
| RANDOM
|
- Due to time constraints there was not enough time to run these samples, they will be tested tomorrow.
|
|
Project name
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