User:Elaine Marie Robbins/Notebook/Capstone/2012/06/01

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  1. Add 1 μL of diluted thiol-modified TS primer to 5.6 mL of citrate AuNP solution.
  2. Mix 3 μL AuNP/TS primer, 0.5 μL 50x dNTP mix, 16 μL sterile water, 2.5 μL 10x PCR buffer, and 2 μL cell extract in a PCR tube.
  3. Incubate in heat block at 30°C for 30 min.
  4. Add 6.10 μL molecular beacon.
  5. Incubate in heat block at 37°C for 1 hr.