User:Elaine Marie Robbins/Notebook/CHEM-496/2011/11/15

From OpenWetWare
Jump to: navigation, search
BDLlogo notext lr.png Properties of AuNP Report.pngMain project page
Resultset previous.pngPrevious entry      Next entryResultset next.png

Objective

To try to stop the unfolding process of BSA and to determine in what situations BSA/AuNP fibers are stable.

Description

  1. Make two solutions of AuNP.
    • Solution 1 (aqueous AuNP):
      1. Combine 0.516 mL of 30 μM BSA and 0.350 mL of 4.17 mM HAuCl4 in a test tube.
      2. Place in 80°C oven until solution turns purple.
    • Solution 2 (AuNP/BSA fibers):
      1. Combine 0.516 mL of 30 μM BSA and 0.700 mL of 4.17 mM HAuCl4 in a test tube.
      2. Place in 80°C oven for 2 hr.
  2. Remove solution 1 from oven.
  3. Add 100 μL of 5.02 mM tris (pH 10.1) to 1 mL of solution 1.
  4. Take UV vis data.
  5. Repeat steps 3 and 4.

Data

Absorbance vs wavelength 11-15-11.jpg


Absorbance vs wavelength magnified 11-15-11.jpg

Notes

Solution 1 began to turn purple after 1.5 hr in the oven, at which point it was removed.

Small purple fibers formed in solution 2.