User:Elaine Marie Robbins/Notebook/CHEM-496/2011/11/15

Properties of AuNP

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Objective To try to stop the unfolding process of BSA and to determine in what situations BSA/AuNP fibers are stable. Description Make two solutions of AuNP. Solution 1 (aqueous AuNP): Combine 0.516 mL of 30 μM BSA and 0.350 mL of 4.17 mM HAuCl4 in a test tube. Place in 80°C oven until solution turns purple. Solution 2 (AuNP/BSA fibers): Combine 0.516 mL of 30 μM BSA and 0.700 mL of 4.17 mM HAuCl4 in a test tube. Place in 80°C oven for 2 hr. Remove solution 1 from oven. Add 100 μL of 5.02 mM tris (pH 10.1) to 1 mL of solution 1. Take UV vis data. Repeat steps 3 and 4. Data Notes Solution 1 began to turn purple after 1.5 hr in the oven, at which point it was removed. Small purple fibers formed in solution 2.