User:Eduardo Vladimir Munoz/Notebook/UNAM Genomics Mexico 2011/2011/09/24

Project name

Main project page Previous entry

High Stabilitiy Plasmid Development Abstract Plasmid extraction of the B0015 dephosphated and repC ligation Gels to corroborate the identity of strains 2, 10 and 11 Extractions Two strains (labeled 3 and 5) were inoculated into liquid LB media and grown for plasmid extraction. Electrophoresis gels The digestions gels had not enough concentration to corroborate the positives. Yet, the PCR amplification revealed that the repC region is only contained in strains 2 and 10. 1 Ladder 500bp 2 C+ PCR 3 C- 4 strain 2 True Positive 5 strain 10 True Positive 6 strain 11 False Positive 1 Ladder 500pb 2 B0015 in pSB1AK3 3 B0015 in pSB1AK3 EcoRV 4 B0015 in pSB1AK3 XhoI 5 repCter strain 2 6 repCter strain 2 EcoRV 7 repCter strain 2 XhoI 8 repCter strain 10 9 repCter strain 10 EcoRV 10 repCter strain 10 XhoI 11 repCter strain 11 12 repCter strain 11 EcoRV 13 repCter strain 11 XhoI 14 15 1 Aarón sample 2 Fermentas ladder 3 B0015 in pSB1AK3 4 B0015 in pSB1AK3 EcoRV 5 B0015 in pSB1AK3 XhoI 6 repCter strain 2 7 repCter strain 2 EcoRV 8 repCter strain 2 XhoI 9 repCter strain 10 10 repCter strain 10 EcoRV 11 repCter strain 10 XhoI 12 repCter strain 11 13 repCter strain 11 EcoRV 14 repCter strain 11 XhoI 15