User:Eduardo Vladimir Munoz/Notebook/UNAM Genomics Mexico 2011/2011/09/23

From OpenWetWare
Jump to: navigation, search
Owwnotebook icon.png Project name Report.pngMain project page
Resultset previous.pngPrevious entry      Next entryResultset next.png

High Stabilitiy Plasmid Development

Abstract

  • Digestions for corroborating the repCter ligation

Digestions

Digestions with EcoRV (a site possessed by the repC locus) and XhoI (a site present in pSB1AK3) were performed on the plasmid extractions of strains 2, 10 and 11, the candidate true positives, for corroboration, as well as on the plasmid without the locus.

EcoRV XhoI
EcoRV 0.5μL XhoI 0.5μL
React 2 1μL React 2 1μL
Sample 4μL Sample 4μL
H2O mQ 4.5μL H2O mQ 4.5μL
Total 10μL Total 10μL