User:Eduardo Vladimir Munoz/Notebook/UNAM Genomics Mexico 2011/2011/09/23

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High Stabilitiy Plasmid Development


  • Digestions for corroborating the repCter ligation


Digestions with EcoRV (a site possessed by the repC locus) and XhoI (a site present in pSB1AK3) were performed on the plasmid extractions of strains 2, 10 and 11, the candidate true positives, for corroboration, as well as on the plasmid without the locus.

EcoRV XhoI
EcoRV 0.5μL XhoI 0.5μL
React 2 1μL React 2 1μL
Sample 4μL Sample 4μL
H2O mQ 4.5μL H2O mQ 4.5μL
Total 10μL Total 10μL