User:Eduardo Vladimir Munoz/Notebook/UNAM Genomics Mexico 2011/2011/09/14

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High Stabilitiy Plasmid Development

Abstract

  • Transformation with new ligations of P1004 with B0014 in pSB1AK3
  • Plasmid extraction corroboration digestions and gel


Transformation

Using yesterdays ligations I transformed competent DH5α cells. I will look for colonies bearing the plasmid tomorrow after their growth.


Extraction corroboration

ElectrophoresisGelSep14 2011PlasmidExtractionAndSimpleDigestionSpeI repC.JPG
Lane Sample
1. 250bp Ladder
2-6 Digestions with SpeI of plasmid extractions of strains 1, 2, 5, 6, 9.
7-11 Plasmid extractions of strains 1, 2, 5, 6, 9.