User:Eduardo Vladimir Munoz/Notebook/UNAM Genomics Mexico 2011/2011/09/01

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High Stability Plasmid Development

Abstract

  • Double digestions of B0014, B1007, P1004

Double Digtestions DD

The following three DD were performed to fill a total volume of 100μL each:

B0014 Bidirectional Terminator

B0014 in pSB1AK3 86μL
BSA 100x 1μL
Buffer 4 10μL
EcoRI-HF 1.5μL
XbaI 1.5μL
Total 100μL


B1007 Bidirectional Terminator

B1007 in pSB1AK3 86μL
BSA 100x 1μL
Buffer 4 10μL
EcoRI-HF 1.5μL
XbaI 1.5μL
Total 100μL


P1004 Chloramphenicol Resistance

P1004 in pSB1A1 86μL
BSA 100x 1μL
Buffer 2 10μL
EcoRI-HF 1.5μL
SpeI 1.5μL
Total 100μL