User:Eduardo Vladimir Munoz/Notebook/UNAM Genomics Mexico 2011/2011/07/30

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Anderson Collection Plasmids Extractions

Abstract

Results Gel Runs for corroborating digestions for the plasmid extractions of July 27.

Yesterday I ran three corroborating gels. These are their results.

Gel 1

1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15.
100A 100B 100C 100D 101A 101B 101C 101D 102A 102B 102D 103A 103C 103D Ladder
I D D DB DB DB D DB DB DB DB DB DB D 2μL


Gel 2

1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15.
104B 104C 104D 105A 105B 105C 105D 106A 106B 106C 106D 107A 107B Ladder 107C
C CA CA CA CA CA C CA MC CA CA CA CA 2μL CA


Gel 3

1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. 18. 19. 20.
107D 108A 108D 109A 110A 110B 110C 110D 111B 111C 112B 112C 113A 113B 114C 115D 116B 116C 116D Ladder
CA CA C CA C C C C I I C I C CA CA I C C C 2μL

Code:

  • C: clean
  • A: abundant (more concentrated than our ladder strong bands)
  • D: dirty
  • B: barely visible
  • I: Invisible
  • M: isomorfical bands present.