User:Eduardo Vladimir Munoz/Notebook/UNAM Genomics Mexico 2011/2011/07/29

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Anderson Collection Plasmids Extractions

Abstract

Corroboration digestions (and gel runs) for the plasmid extractions of July 27.

Having selected yesterday the plasmid extractions that worked properly, Melissa and I digested them with EcoRI. We have 53 digestions, corresponding to those extractions whic were clean and concentrated enough. I ran gels to corroborate these extractions quality with the digestions. One single bar indicates that the plasmid is both clean and present (at aproximately 3kb). My gel columns are:

Gel 1

1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15.
100A 100B 100C 100D 101A 101B 101C 101D 102A 102B 102D 103A 103C 103D Ladder


Gel 2

1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15.
104B 104C 104D 105A 105B 105C 105D 106A 106B 106C 106D 107A 107B Ladder 107C


Gel 3

1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. 18. 19. 20.
107D 108A 108D 109A 110A 110B 110C 110D 111B 111C 112B 112C 113A 113B 114C 115D 116B 116C 116D Ladder

Tomorrow I will dye these three gels.