User:Eamonn B. Mallon/Notebook/Monoparental expression in worker reproduction genes/2010/06/14

From OpenWetWare
Jump to: navigation, search
Owwnotebook icon.png Project name Report.pngMain project page
Resultset previous.pngPrevious entry      

Monoparental expression in worker reproduction genes

Basic protocol for production of reproducing workers.
Mark callows from colony A. Put back in nest for 2 days. Once 2 days old add one worker from colony A plus two callows from B plus some young cocoons. Leave for 7 days. Older worker can assumed to be egg laying. Verify with pictures taken on addition and at 7 days (check that the brood pile has been added to. Store all bees at -80oC

Everyday observation for competition point

5 minutes scanning looking for (1) Worker oviposition (2) oophagy (3) egg cell detruction (4) two or more opened egg cells over 2 or more successive days.