User:Eamonn B. Mallon/Notebook/Monoparental expression in worker reproduction genes/2010/06/03
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Monoparental expression in worker reproduction genes
Preparation of reproducing workers is achieved as follows: five callow workers (within 24 h of emergence) from the same colony will be marked with numbered tags and kept in their natal colony for 2 additional days. Thereafter, they will be removed from the nest and placed separately with two 1-day-old workers from different donor colonies, creating groups of three queenless workers. Young cocoons will act as a substrate for cell construction. The oldest worker will produce males.