User:Douglas M. Fox/Notebook/AU CHEM-571 F2011 Lab Support/2017/09/12

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Tasklist Prepare 0.1 M buffer solutions of pH 10, 11, & 12 I suggest using Na2HPO4/Na3PO4 or NaHCO3/Na2CO3. Prepare your Au/AA solutions using buffer instead of water for all three AA Make a 10 mM HAuCl4 solution and a 100 mM AA solution (using your basic R group) Prepare AuNP solutions using 10:1, 1:1, & 1:10 Au:AA at each pH Set aside your 18 AuNP preparation solutions for overnight heating Prepare Buffer for Bradford Analysis 1L 50mM Tris 50mM NaCl pH 7.5 Prepare Buffer for Dialysis 100 mL Glycine Buffer with pH = 3.5 Prepare Buffer for SDS-PAGE 3.75mL 0.5M Tris pH 6.8 ---> 6.1042 g Tris in 100mL water ---> 0.504M Tris 15.0mL of 50% glycerol ---> 7.5mL glycerol + 7.5mL water 0.3ml of 1% Bromphenol Blue ---> 0.009g Bromphenol Blue in 1mL water 6.0mL 10% Sodium Dodecyl Sulfate (SDS) ---> 1g SDS in 10mL water H2O to make 30mL total