User:Douglas M. Fox/Notebook/AU CHEM-571 F2011 Lab Support/2014/11/11

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  • Continue Dialysis
  • Polarization Fluorescence
    • Prepare tryptophan solution such that fluorescence intensity is between 600 and 800 when λex = 280 nm
    • Prepare enzyme solutions of same concentration
    • Use BSA, lysozyme, HRP, Pepcin, and Lipase
    • Measure fluorescence using no filter, vertical emission filter, and horizontal emission filter
    • Plot fluorescence intensities vs. molar mass of proteins/tryptophan
    • Plot (vert - horiz)/(vert + 2*horiz) vs. molar mass of proteins/tryptophan