User:Douglas M. Fox/Notebook/AU CHEM-571 F2011 Lab Support/2014/11/04

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Tasklist Measure dialysis concentrations from last week Prepare a new dialysis using colloid solution and CaCl2 or HCl For those running CaCl2, use 3500 MWCO & test concentrations between 10 and 50 mM For those running HCl, use 25000 MWCO & test concentrations between 100 μM and 10 mM Prepare solutions for enzyme kinetics Prepare 25 mL 66 mM Potassium phosphate, pH 6.24 (beaker prep is fine) Place half in a Falcon tube, label & refrigerate overnight Prepare 10 mL 0.01 wt% Micrococcus lysodeikticus Cell Suspension (in freezer) using phosphate buffer Adjust the cell suspension concentration until the Absorbance at λ = 450 nm is between 0.6 & 0.7 The full protocol is here