# User:Dominique Brager/Notebook/481 Notebook/2016/10/12

Biomaterials Design Lab Main project page
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## Objective

Continue running 20% column and 30% column. Pump is used on 30% with a speed of 50mL in 9:45 minutes. The pipette bulb is used on the 20% to speed it up. Because it has to be hand pumped and it's inconsistent, it's hard to determine a rate.

Finished running UV-Vis on the 10% clay beads. Samples 31-34 were of 20mL.

## Description

Ran data analysis on AC.

• Found the Max Absorbance for each run.
• Using the Beer's Law calibration Curve we found a few weeks ago (y=0.0813x) calculated concentration for each sample.
• Created plot of concentration vs. Volume Eluted.
• Calculated the amount of MG added to the Column
7 samples *5mL/sample*1L/1000mL*50mg/1L = 1.75mg
• Calculated the amount of MG eluted.
From samples 1-7:
5mL*1L/1000mL*concentration of sample = mg of MG not adsorbed
For sample 8:
1mL*1L/1000mL*concentration of sample = mg of MG not adsorbed
4mL*1L/1000mL*concentration of sample = mg of MG desorbed
For samples 9-108:
5mL*1L/1000mL*concentration of sample = mg of MG desorbed
• Subtract mg of MG not adsorbed from amount of MG added to column to get amount of MG adsorbed by the column.
• Find capacity of activated carbon by dividing mass of MG adsorbed by mass of activated carbon in beads.

## Data

Graph of Concentration vs. Volume Eluted for the Activated Carbon.

Amount desorbed: 0.992mg

Mass of Beads in Column: 1.8075g